Atsiliepimai
Aprašymas
Part I. Murine Skeletal Development, Repair, and Genetic Models
1. Overview of Skeletal Development
Tatsuya Kobayashi and Henry M. Kronenberg
2. Overview of Skeletal Repair (Fracture Healing and Its Assessment)
Elise F. Morgan, Anthony De Giacomo, and Louis C. Gerstenfeld
3. Advantages and Limitations of Cre Mouse Lines Used in Skeletal Research
Florent Elefteriou and Greig Couasnay
Part II. Skeletal Repair, Parabiosis, Transplantations, and Organ Cultures
4. Generation of Closed Transverse Fractures in Small Animals
Anthony De Giacomo, Elise F. Morgan, and Louis C. Gerstenfeld
5. A Mouse Femoral Ostectomy Model to Assess Bone Graft Substitutes
Ryan Trombetta, Emma K. Knapp, and Hani A. Awad
6. Surgical Induction of Posttraumatic Osteoarthritis in the Mouse
Robert D. Maynard, David A. Villani, William G. Schroeder, Douglas J. Adams, and Michael J. Zuscik
7. Parabiosis: Assessing the Effects of Circulating Cells and Factors on the Skeleton
Benjamin Aaron Alman and Gurpreet Singh Baht
8. Murine Limb Bud Organ Cultures for Studying Musculoskeletal Development
Martin Arostegui and T. Michael Underhill
9. Murine Limb Explant Cultures to Assess Cartilage Development
Manuela Wuelling and Andrea Vortkamp
10. Renal Capsule Transplantation to Assay Angiogenesis in Skeletal Development and Repair
Anais Julien, Simon Perrin, Rana Abou-Khalil, and Céline Colnot
Part III. Structural, Histological, and Molecular Analyses on Skeletal Tissues and Tissue Sections
11. MicroCT for Scanning and Analysis of Mouse Bones
Yung Kim, Michael D. Brodt, Simon Y. Tang, and Matthew J. Silva12. Four-Point Bending Testing for Mechanical Assessment of Mouse Bone Structural Properties
Hattie C. Cutcliffe and Louis E. DeFrate
13. Phenotyping Intact Mouse Bones using Bone CLARITY
Jennifer B. Treweek, Aidan Beres, Nathan Johnson, and Alon Greenbaum
14. Processing and Sectioning Undecalcified Murine Bone Specimens
Thomas B. Bemenderfer, Jonathan S. Harris, Keith W. Condon, Jiliang Li, and Melissa A. Kacena
15. Preparation of Thin Frozen Sections from Nonfixed And Undecalcified Hard Tissues Using Kawamoto's Film Method (2020)
Tadafumi Kawamoto and Komei Kawamoto
16. Demineralized Murine Skeletal Histology
Anthony J. Mirando and Matthew J. Hilton
17. Assessment of Osteocytes: Techniques for Studying Morphological and Molecular Changes Associated With Perilacunar/ Canalicular Remodeling Of the Bone Matrix
Neha S. Dole, Cristal S. Yee, Charles A. Schurman, Sarah L. Dallas, and Tamara Alliston
18. Cell Lineage Tracing: Co-localization of Cell Lineage Markers with a Fluorescent Reporter
Yan Jing, Patricia Simmer, and Jian Q. Feng
19. Immunofluorescent Staining of Adult Murine Paraffin-embedded Skeletal Tissue
Neta Felsenthal and Elazar Zelzer
20. Detection of Hypoxic Regions in the Bone Microenvironment
Wendi Guo and Colleen Wu
21. EdU-based Assay of Cell Proliferation and Stem Cell Quiescence in Skeletal Tissue Sections
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Part I. Murine Skeletal Development, Repair, and Genetic Models
1. Overview of Skeletal Development
Tatsuya Kobayashi and Henry M. Kronenberg
2. Overview of Skeletal Repair (Fracture Healing and Its Assessment)
Elise F. Morgan, Anthony De Giacomo, and Louis C. Gerstenfeld
3. Advantages and Limitations of Cre Mouse Lines Used in Skeletal Research
Florent Elefteriou and Greig Couasnay
Part II. Skeletal Repair, Parabiosis, Transplantations, and Organ Cultures
4. Generation of Closed Transverse Fractures in Small Animals
Anthony De Giacomo, Elise F. Morgan, and Louis C. Gerstenfeld
5. A Mouse Femoral Ostectomy Model to Assess Bone Graft Substitutes
Ryan Trombetta, Emma K. Knapp, and Hani A. Awad
6. Surgical Induction of Posttraumatic Osteoarthritis in the Mouse
Robert D. Maynard, David A. Villani, William G. Schroeder, Douglas J. Adams, and Michael J. Zuscik
7. Parabiosis: Assessing the Effects of Circulating Cells and Factors on the Skeleton
Benjamin Aaron Alman and Gurpreet Singh Baht
8. Murine Limb Bud Organ Cultures for Studying Musculoskeletal Development
Martin Arostegui and T. Michael Underhill
9. Murine Limb Explant Cultures to Assess Cartilage Development
Manuela Wuelling and Andrea Vortkamp
10. Renal Capsule Transplantation to Assay Angiogenesis in Skeletal Development and Repair
Anais Julien, Simon Perrin, Rana Abou-Khalil, and Céline Colnot
Part III. Structural, Histological, and Molecular Analyses on Skeletal Tissues and Tissue Sections
11. MicroCT for Scanning and Analysis of Mouse Bones
Yung Kim, Michael D. Brodt, Simon Y. Tang, and Matthew J. Silva12. Four-Point Bending Testing for Mechanical Assessment of Mouse Bone Structural Properties
Hattie C. Cutcliffe and Louis E. DeFrate
13. Phenotyping Intact Mouse Bones using Bone CLARITY
Jennifer B. Treweek, Aidan Beres, Nathan Johnson, and Alon Greenbaum
14. Processing and Sectioning Undecalcified Murine Bone Specimens
Thomas B. Bemenderfer, Jonathan S. Harris, Keith W. Condon, Jiliang Li, and Melissa A. Kacena
15. Preparation of Thin Frozen Sections from Nonfixed And Undecalcified Hard Tissues Using Kawamoto's Film Method (2020)
Tadafumi Kawamoto and Komei Kawamoto
16. Demineralized Murine Skeletal Histology
Anthony J. Mirando and Matthew J. Hilton
17. Assessment of Osteocytes: Techniques for Studying Morphological and Molecular Changes Associated With Perilacunar/ Canalicular Remodeling Of the Bone Matrix
Neha S. Dole, Cristal S. Yee, Charles A. Schurman, Sarah L. Dallas, and Tamara Alliston
18. Cell Lineage Tracing: Co-localization of Cell Lineage Markers with a Fluorescent Reporter
Yan Jing, Patricia Simmer, and Jian Q. Feng
19. Immunofluorescent Staining of Adult Murine Paraffin-embedded Skeletal Tissue
Neta Felsenthal and Elazar Zelzer
20. Detection of Hypoxic Regions in the Bone Microenvironment
Wendi Guo and Colleen Wu
21. EdU-based Assay of Cell Proliferation and Stem Cell Quiescence in Skeletal Tissue Sections
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