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Oxidative stress lies at the crossroads of redox biology, metabolism, and chronic disease. This book provides an integrated and mechanistic exploration of reactive oxygen species (ROS), emphasizing their dual role as essential signaling molecules and drivers of cellular injury when redox balance is disrupted. Beginning with the biological origins of ROS, including mitochondrial and endoplasmic reticulum sources. Subsequent chapters examine antioxidant systems and the principles governing redox homeostasis. Building on the concept of the redox code, the book links redox dysregulation to metabolic disorders such as diabetes, obesity, and metabolic syndrome, highlighting the roles of gut microbiota, lipid oxidation, mitochondrial dysfunction, and oxidative stress in non-alcoholic fatty liver disease.