Knygos.lt nariams
Knygos.lt nariams
- Išsiųsime per 12–18 d.d.
/images/books/13375878/9783838124278.jpg)
- Autorius: Anna Taubenberger
- Leidėjas: Sudwestdeutscher Verlag Fur Hochschulschriften AG
- Metai: 2011
- Puslapiai: 184
- ISBN-10: 3838124278
- ISBN-13: 9783838124278
- Formatas: 15.2 x 22.9 x 1.1 cm, minkšti viršeliai
- Kalba: Anglų
Quantifying Cell-Ecm Adhesion by Single Cell Force Spectroscopy (el. knyga) (skaityta knyga) | knygos.lt
Atsiliepimai
Aprašymas
Integrin-mediated adhesive interactions of cells with the extracellular matrix (ECM) regulate important cellular functions and play a critical role in disease. Therefore, studying the mechanisms underlying integrin-binding and regulation is of great interest. In the presented work three respective biological questions have been addressed using Atomic Force Microscopy based Single Cell Force Spectroscopy (AFM-SCFS). The work is split into six chapters. The first chapter provides an introduction into cell-ECM interactions. Thereafter different adhesion assays are compared and contrasted, and the theoretical Bell-Evans model is discussed. In chapter four, alpha2beta1-integrin mediated cell adhesion to collagen I is quantified. Thereafter the effects of the exposure of cryptic integrin binding sites within collagen I on pre-osteoblasts are analysed. At last, the role of BCR/ABL in modulating myeloid progenitor cell adhesion to stromal cells and ECM-coated substrates is studied. Taken together, the work demonstrates that AFM-SCFS is a versatile tool that permits monitoring of cell adhesion from single-molecule recognition events to the formation of more complex adhesion sites.
- Autorius: Anna Taubenberger
- Leidėjas: Sudwestdeutscher Verlag Fur Hochschulschriften AG
- Metai: 2011
- Puslapiai: 184
- ISBN-10: 3838124278
- ISBN-13: 9783838124278
- Formatas: 15.2 x 22.9 x 1.1 cm, minkšti viršeliai
- Kalba: Anglų
Integrin-mediated adhesive interactions of cells with the extracellular matrix (ECM) regulate important cellular functions and play a critical role in disease. Therefore, studying the mechanisms underlying integrin-binding and regulation is of great interest. In the presented work three respective biological questions have been addressed using Atomic Force Microscopy based Single Cell Force Spectroscopy (AFM-SCFS). The work is split into six chapters. The first chapter provides an introduction into cell-ECM interactions. Thereafter different adhesion assays are compared and contrasted, and the theoretical Bell-Evans model is discussed. In chapter four, alpha2beta1-integrin mediated cell adhesion to collagen I is quantified. Thereafter the effects of the exposure of cryptic integrin binding sites within collagen I on pre-osteoblasts are analysed. At last, the role of BCR/ABL in modulating myeloid progenitor cell adhesion to stromal cells and ECM-coated substrates is studied. Taken together, the work demonstrates that AFM-SCFS is a versatile tool that permits monitoring of cell adhesion from single-molecule recognition events to the formation of more complex adhesion sites.
Atsiliepimai