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The book aims to shed light on some of the unexplored aspects of cryobiology. It discusses thoroughly the various basic and fundamental concepts of this subject. Cryobiology, as part of biology deals with the study of changes occurring in living beings present in cryosphere of the atmosphere, when they are subjected to very low temperature. The systems studied in this field are organs, proteins, tissues, cells, and organisms. This text presents the complex subject of cryobiology in the most comprehensible and easy to understand language. It explores all the important aspects of the area in the present day scenario. This textbook is meant for students who are looking for an elaborate reference text on cryobiology.