Hedgehog Signaling + nemokamas atvežimas! | knygos.lt

Aprašymas

1. Generation and Analysis of Mosaic Spinal Cord Organoids Derived from Mouse Embryonic Stem Cells

Carina Jägers and Henk Roelink

2. Evaluating Hedgehog Acyltransferase Activity and Inhibition Using the Acylation-Coupled Lipophilic Induction of Polarization (Acyl-cLIP) Assay

Sebastian A. Andrei, Thomas Lanyon-Hogg, and Edward W. Tate

3. Analysis of Protein Cholesterylation by Biorthogonal Labeling

Ao Hu, Meng Zhou, and Bao-Liang Song

4. An In Situ Fluorescence Assay for Cholesterol Transporter Activity of the Patched

Wonhwa Cho, Arthur Ralko, and Ashutosh Sharma

5. Imaging of the Ciliary Cholesterol Underling the Sonic Hedgehog Signal Transduction

Tatsuo Miyamoto, Kosuke Hosoba, Silvia Natsuko Akutsu, and Shinya Matsuura

6. Studying Hedgehog Signaling during Mouse Neural Tube Development

Sandii Constable, Kevin White, Bandarigoda Nipunika Somatilaka, and Saikat Mukhopadhyay

7. Measuring and Manipulating Membrane Cholesterol for the Study of Hedgehog Signaling

Maia Kinnebrew, Kristen A. Johnson, Arun Radhakrishnan, and Rajat Rohatgi

8. The Immunofluorescence-Based Detection of Hedgehog Pathway Components in Primary Cilia of Cultured Cells

Shu Yang and Xiaoyan Zheng

9. Analysis of Dispatched Protein Processing and Sonic Hedgehog Ligand Release

Elizabeth Cleverdon, Daniel P. Stewart, and Stacey K. Ogden

10. Expression, Purification, and Structure Determination of Human PTCH1-HH-N Complexes

Xiaofeng Qi, Philip Schmiege, Leticia Esparza, and Xiaochun Li

11. Characterization of Smoothened Phosphorylation and Activation

Jianhang Jia, Yuhong Han, Yong Suk Cho, and Jin Jiang

12. Cell-Based Assays for Smoothened Ubiquitination and Sumoylation

Yuhong Han and Jin Jiang

13. Biochemical Assays to Directly Assess Smoothened Activation by a Conformationally Sensitive Nanobody

Ishan Deshpande and Aashish Manglik

14. Studies of SMOOTHENED Activation in Cell-Free and Reconstituted Systems

Isaac B. Nelson and Benjamin R. Myers

15. Rapid, Direct SMOOTHENED Activity Assays in Live Cells Using cAMP-Based Conformational Sensors

Madison F. Walker and Benjamin R. Myers

16. Light-Induced Differentiation of Forebrain Organoids by NVOC-SAG

Ryuji Misawa and Yoshiho Ikeuchi

17. NanoBRET and NanoBiT/BRET-Based Ligand Binding Assays Permit Quantitative Assessment of Small Molecule Lig